Healthcare facilities are consistently connected to a range of potential protection problems worldwide. Non-compliance with hand hygiene is a significant problem in healthcare. The objectives of the study were to assess the knowledge, practice and resources for compliance scores on hand hygiene among health care workers; correlate knowledge, practice and resources for compliance scores on hand hygiene among health care workers; and associate the level of knowledge, practice and resources for compliance on hand hygiene with selected demographic variables. A descriptive survey was used for the present study. The target population of the study was health care workers. The investigator approached around 10 health care organisations across India seeking permission, and 4 of them responded. The sampling technique solicited was snow snowball method. The tool, prepared in the form of a Google form, had three sections apart from demographic variables. The generated Google link was shared with the Heads of the institutions. Participants who were willing to participate for the study completed the self-reports. The calculated sample size was 423. However, a total of 500 participants responded within the time frame. The tool was tested for reliability, and the values ranged from 0.81 to 0.86. The results showed that 95 percent of participants reported that resources were available for compliance, 51.7 percent reported having practised hand hygiene, and 53.8 percent knew it. A significant correlation was found between resources for compliance and knowledge (p<0.001). No significant association was found with demographic variables. It was concluded that strict observations, regular audits and added disciplinary measures to persuade the health care workers to comply with hand hygiene are essential.