The study aims to assess the knowledge on pre-term delivery among pregnant women, and to find out the association between knowledge on pre-term delivery among pregnant women and selected variables. The setting was, outpatient department in the Institute of Maternal and Child Health, Kozhikode (Kerala). The conceptual framework was based on the revised health promotion model by Nola J Pender in 2006, which describes the multi-dimensional nature of a person as they interact with their environment to pursue health. In this study, which used descriptive survey design with a consecutive sampling technique, data were collected from 350 pregnant women. Semi-structured interview schedule was used to assess the knowledge on pre-term delivery. The study found that 32.6 percent pregnant women had poor knowledge, whereas 47.1 percent had average knowledge, and only 20.3 percent had good knowledge on pre-term delivery. Score obtained from the pregnant women on knowledge of pre-term delivery was 13, and maximum 101; the mean score was 49.94, and standard deviation 21.135. Computed p-value was statistically significant at 0.05 level of significance for knowledge on preterm delivery. Hence, the null hypothesis was not accepted. It can be interpreted that there is an association between knowledge on pre-term delivery with age, education, occupation, occupation of husband and history of pre-term delivery.