Monkey pox (mpox), a re-emerging zoonotic viral disease is posing a significant public health challenge, particularly in low- and middle-income countries. Adequate knowledge and a favourable attitude among healthcare professionals and learners are essential for effective prevention and control. A cross-sectional study was conducted in 2025 among 332 participants, 166 healthcare professionals (HCPs) and 166 learners of healthcare profession curricula (HCPC), in a tertiary care hospital in Bhopal (MP). Data were collected using a structured knowledge questionnaire and an attitude scale. Knowledge level was ‘Good’ among 30.7 percent of HCPs and 27.7 percent of learners. A favourable attitude towards mpox prevention and management was reported by 76.5 percent of HCPs compared to 13.9 percent of learners. Among HCPs, attitude was significantly associated with age and prior experience of caring for a mpox patient, while among learners, knowledge was associated with gender and programme/course, and attitude with programme/course. A moderate positive correlation was observed among HCPs (rho = 0.431, p < 0.001), whereas a weak but statistically significant correlation was found among learners (rho = 0.200, p = 0.01). The findings indicate that although HCPs demonstrated better knowledge and attitudes than learners, gaps remain, particularly due to limited exposure to training programmes or lack of integration of mpox prevention measures in curriculum. Strengthening curriculum and implementing regular training programmes on mpox are essential to improve preparedness for mpox prevention and management.